The lab says fixation will trigger the dyes to leak from the cells. I feel the assumption is the fact that fixation may perhaps hurt cell membranes. I did discover a paper which describes fixation utilizing methanol or acetic acid, soon after staining by using a DCFDA spinoff. DCFDA is https://archersdnwf.blogdomago.com/28701396/not-known-facts-about-2-fdck-hcl