The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions ended up digested and cloned right into a pET21a+ vector working with NdeI and XhoI restriction enzymes. A point mutation was released by the https://cashxrkbt.blog-gold.com/32626291/everything-about-arv-471